Anti-ApoER2 Monoclonal Antibody

Product Name

Anti-ApoER2 Monoclonal Antibody

View all Apolipoprotein E R2/ApoE R2 Antibodies

SKU/Catalog Number

M03444

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ApoER2 Monoclonal Antibody catalog # M03444. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ApoER2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03444)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACFH-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ApoER2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03444 is reactive to LRP8 in Human, Mouse, Rat

Applications

M03444 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

106 kDa

Calculated molecular weight

105.634kDa

Background of Apolipoprotein E R2/ApoE R2

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ApoER2 using anti-ApoER2 antibody (M03444).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ApoER2 antigen affinity purified monoclonal antibody (Catalog # M03444) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ApoER2 at approximately 106 kDa. The expected band size for ApoER2 is at 106 kDa.

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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

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Western blot analysis of ApoER2 expression in C6 cell lysate.

Gene/Protein Information For LRP8 (Source: Uniprot.org, NCBI)

Alternative Names

ApoER2; APOER2HSZ75190; Apolipoprotein E R2; Apolipoprotein E receptor 2; low density lipoprotein receptor-related protein 8, apolipoprotein e receptor; low-density lipoprotein receptor-related protein 8; Lr8b; LRP8; LRP-8; MCI1 LRP8 APOER2, HSZ75190, LRP-8, MCI1 LDL receptor related protein 8 low-density lipoprotein receptor-related protein 8|ApoE receptor 2|low density lipoprotein receptor-related protein 8, apolipoprotein e receptor

For more info on LRP8, check out the LRP8 Infographic

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In this infographic, you will see the following information for LRP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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